Construct: shRNA TRCN0000335772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008115.2-1232s21c1
DNA Barcode:
CCTGAACGACAACTGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gfra2 (14586)
Gene Description:
glial cell line derived neurotrophic factor family receptor alpha 2
Transcript:
RefSeq NM_008115.2 (NON-CURRENT)
Match location:
Position 1232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAACGACAACTGCAAGAA
Hairpin Sequence:
5'-CCGG-CCTGAACGACAACTGCAAGAA-CTCGAG-TTCTTGCAGTTGTCGTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAACGACAACTGCAAGAACTCGAGTTCTTGCAGTTGTCGTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAACGACAACTGCAAGAACTCGAGTTCTTGCAGTTGTCGTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000079139