Construct: shRNA TRCN0000335810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005356.3-1417s21c1
DNA Barcode:
GGGATCCTGCTGACGGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LCK (3932)
Gene Description:
LCK proto-oncogene, Src family tyrosine kinase
Transcript:
RefSeq NM_005356.3 (NON-CURRENT)
Match location:
Position 1417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGATCCTGCTGACGGAAATT
Hairpin Sequence:
5'-CCGG-GGGATCCTGCTGACGGAAATT-CTCGAG-AATTTCCGTCAGCAGGATCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGATCCTGCTGACGGAAATTCTCGAGAATTTCCGTCAGCAGGATCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGATCCTGCTGACGGAAATTCTCGAGAATTTCCGTCAGCAGGATCCC-3'

Other clones with same target sequence:

(none)