Construct: shRNA TRCN0000335909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000790.3-1686s21c1
DNA Barcode:
GCTTAGTATCTCATCAACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDC (1644)
Gene Description:
dopa decarboxylase
Transcript:
RefSeq NM_000790.3 (NON-CURRENT)
Match location:
Position 1686 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGTATCTCATCAACAAA
Hairpin Sequence:
5'-CCGG-GCTTAGTATCTCATCAACAAA-CTCGAG-TTTGTTGATGAGATACTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGTATCTCATCAACAAACTCGAGTTTGTTGATGAGATACTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGTATCTCATCAACAAACTCGAGTTTGTTGATGAGATACTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000005524