Construct: shRNA TRCN0000335917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017547.2-398s21c1
DNA Barcode:
GCAGTTCTCATTGCCTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXRED1 (55572)
Gene Description:
FAD dependent oxidoreductase domain containing 1
Transcript:
RefSeq NM_017547.2 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTCTCATTGCCTGAGAA
Hairpin Sequence:
5'-CCGG-GCAGTTCTCATTGCCTGAGAA-CTCGAG-TTCTCAGGCAATGAGAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTCTCATTGCCTGAGAACTCGAGTTCTCAGGCAATGAGAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTCTCATTGCCTGAGAACTCGAGTTCTCAGGCAATGAGAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000005562