Construct: shRNA TRCN0000335961

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017901.4-592s21c1
DNA Barcode:
GCCTACCTCTTTGCACACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPCN1 (53373)
Gene Description:
two pore segment channel 1
Transcript:
RefSeq NM_017901.4 (NON-CURRENT)
Match location:
Position 592 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335961)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACCTCTTTGCACACAAT
Hairpin Sequence:
5'-CCGG-GCCTACCTCTTTGCACACAAT-CTCGAG-ATTGTGTGCAAAGAGGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACCTCTTTGCACACAATCTCGAGATTGTGTGCAAAGAGGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACCTCTTTGCACACAATCTCGAGATTGTGTGCAAAGAGGTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000005042