Construct: shRNA TRCN0000336020

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026228.4-1636s21c1
DNA Barcode:
TACGCAGGAGACATCGAATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc39a8 (67547)
Gene Description:
solute carrier family 39 (metal ion transporter), member 8
Transcript:
RefSeq NM_026228.4 (NON-CURRENT)
Match location:
Position 1636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336020)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGCAGGAGACATCGAATTG
Hairpin Sequence:
5'-CCGG-TACGCAGGAGACATCGAATTG-CTCGAG-CAATTCGATGTCTCCTGCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGCAGGAGACATCGAATTGCTCGAGCAATTCGATGTCTCCTGCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGCAGGAGACATCGAATTGCTCGAGCAATTCGATGTCTCCTGCGTA-3'

Other clones with same target sequence:

(none)