Construct: shRNA TRCN0000336087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012031.3-2554s21c1
DNA Barcode:
GACGTCTCCAAGCCTACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spag1 (26942)
Gene Description:
sperm associated antigen 1
Transcript:
RefSeq NM_012031.3 (CURRENT)
Match location:
Position 2554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGTCTCCAAGCCTACTAAT
Hairpin Sequence:
5'-CCGG-GACGTCTCCAAGCCTACTAAT-CTCGAG-ATTAGTAGGCTTGGAGACGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGTCTCCAAGCCTACTAATCTCGAGATTAGTAGGCTTGGAGACGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGTCTCCAAGCCTACTAATCTCGAGATTAGTAGGCTTGGAGACGTC-3'

Other clones with same target sequence:

(none)