Construct: shRNA TRCN0000336105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175542.3-2573s21c1
DNA Barcode:
CGGTTCGTTCACTAGCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rttn (246102)
Gene Description:
rotatin
Transcript:
RefSeq NM_175542.3 (CURRENT)
Match location:
Position 2573 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTCGTTCACTAGCATTAA
Hairpin Sequence:
5'-CCGG-CGGTTCGTTCACTAGCATTAA-CTCGAG-TTAATGCTAGTGAACGAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTCGTTCACTAGCATTAACTCGAGTTAATGCTAGTGAACGAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTCGTTCACTAGCATTAACTCGAGTTAATGCTAGTGAACGAACCG-3'

Other clones with same target sequence:

(none)