Construct: shRNA TRCN0000336124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145743.2-1793s21c1
DNA Barcode:
CCTTTGTAGATCCGAGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Afg1l (215951)
Gene Description:
AFG1 like ATPase
Transcript:
RefSeq NM_145743.2 (CURRENT)
Match location:
Position 1793 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGTAGATCCGAGTTTAT
Hairpin Sequence:
5'-CCGG-CCTTTGTAGATCCGAGTTTAT-CTCGAG-ATAAACTCGGATCTACAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGTAGATCCGAGTTTATCTCGAGATAAACTCGGATCTACAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGTAGATCCGAGTTTATCTCGAGATAAACTCGGATCTACAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000121012