Construct: shRNA TRCN0000336278

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175155.4-1997s21c1
DNA Barcode:
CGGCACGTTCAAGTTCATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sash1 (70097)
Gene Description:
SAM and SH3 domain containing 1
Transcript:
RefSeq NM_175155.4 (CURRENT)
Match location:
Position 1997 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336278)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCACGTTCAAGTTCATCTA
Hairpin Sequence:
5'-CCGG-CGGCACGTTCAAGTTCATCTA-CTCGAG-TAGATGAACTTGAACGTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCACGTTCAAGTTCATCTACTCGAGTAGATGAACTTGAACGTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCACGTTCAAGTTCATCTACTCGAGTAGATGAACTTGAACGTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000121383