Construct: shRNA TRCN0000336286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025564.2-461s21c1
DNA Barcode:
GTACAAGACTTGAAATGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magohb (66441)
Gene Description:
mago homolog B, exon junction complex core component
Transcript:
RefSeq NM_025564.2 (CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACAAGACTTGAAATGCTTA
Hairpin Sequence:
5'-CCGG-GTACAAGACTTGAAATGCTTA-CTCGAG-TAAGCATTTCAAGTCTTGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACAAGACTTGAAATGCTTACTCGAGTAAGCATTTCAAGTCTTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACAAGACTTGAAATGCTTACTCGAGTAAGCATTTCAAGTCTTGTAC-3'

Other clones with same target sequence:

  1. TRCN0000174912