Construct: shRNA TRCN0000336301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153055.3-441s21c1
DNA Barcode:
TGGTATCGTTTACGGTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec63 (140740)
Gene Description:
SEC63-like (S. cerevisiae)
Transcript:
RefSeq NM_153055.3 (CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTATCGTTTACGGTTATTA
Hairpin Sequence:
5'-CCGG-TGGTATCGTTTACGGTTATTA-CTCGAG-TAATAACCGTAAACGATACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTATCGTTTACGGTTATTACTCGAGTAATAACCGTAAACGATACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTATCGTTTACGGTTATTACTCGAGTAATAACCGTAAACGATACCA-3'

Other clones with same target sequence:

(none)