Construct: shRNA TRCN0000336313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013883.2-1879s21c1
DNA Barcode:
CTCACTGCCACAGAGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scmh1 (29871)
Gene Description:
sex comb on midleg homolog 1
Transcript:
RefSeq NM_013883.2 (CURRENT)
Match location:
Position 1879 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACTGCCACAGAGTATAAT
Hairpin Sequence:
5'-CCGG-CTCACTGCCACAGAGTATAAT-CTCGAG-ATTATACTCTGTGGCAGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACTGCCACAGAGTATAATCTCGAGATTATACTCTGTGGCAGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACTGCCACAGAGTATAATCTCGAGATTATACTCTGTGGCAGTGAG-3'

Other clones with same target sequence:

(none)