Construct: shRNA TRCN0000336443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030730.2-985s21c1
DNA Barcode:
CCATGTGAATGACGCCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad54l2 (81000)
Gene Description:
RAD54 like 2 (S. cerevisiae)
Transcript:
RefSeq NM_030730.2 (CURRENT)
Match location:
Position 985 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGAATGACGCCTTAAA
Hairpin Sequence:
5'-CCGG-CCATGTGAATGACGCCTTAAA-CTCGAG-TTTAAGGCGTCATTCACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGAATGACGCCTTAAACTCGAGTTTAAGGCGTCATTCACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGAATGACGCCTTAAACTCGAGTTTAAGGCGTCATTCACATGG-3'

Other clones with same target sequence:

(none)