Construct: shRNA TRCN0000336451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028658.3-2476s21c1
DNA Barcode:
CCTGGAGACCTTTGAAGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r21 (73825)
Gene Description:
protein phosphatase 1, regulatory subunit 21
Transcript:
RefSeq NM_028658.3 (NON-CURRENT)
Match location:
Position 2476 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGACCTTTGAAGAATA
Hairpin Sequence:
5'-CCGG-CCTGGAGACCTTTGAAGAATA-CTCGAG-TATTCTTCAAAGGTCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGACCTTTGAAGAATACTCGAGTATTCTTCAAAGGTCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGACCTTTGAAGAATACTCGAGTATTCTTCAAAGGTCTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000192443