Construct: shRNA TRCN0000336503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030730.2-4825s21c1
DNA Barcode:
GCAGGCTCTATTGCTGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad54l2 (81000)
Gene Description:
RAD54 like 2 (S. cerevisiae)
Transcript:
RefSeq NM_030730.2 (CURRENT)
Match location:
Position 4825 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCTCTATTGCTGTTCAT
Hairpin Sequence:
5'-CCGG-GCAGGCTCTATTGCTGTTCAT-CTCGAG-ATGAACAGCAATAGAGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCTCTATTGCTGTTCATCTCGAGATGAACAGCAATAGAGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCTCTATTGCTGTTCATCTCGAGATGAACAGCAATAGAGCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000115216