Construct: shRNA TRCN0000336510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028035.4-812s21c1
DNA Barcode:
AGCAGTTCTCATGGATGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx10 (71982)
Gene Description:
sorting nexin 10
Transcript:
RefSeq NM_028035.4 (CURRENT)
Match location:
Position 812 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTTCTCATGGATGTAAA
Hairpin Sequence:
5'-CCGG-AGCAGTTCTCATGGATGTAAA-CTCGAG-TTTACATCCATGAGAACTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTTCTCATGGATGTAAACTCGAGTTTACATCCATGAGAACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTTCTCATGGATGTAAACTCGAGTTTACATCCATGAGAACTGCT-3'

Other clones with same target sequence:

(none)