Construct: shRNA TRCN0000336522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026693.4-481s21c1
DNA Barcode:
TTCCTGCTTGTGTCTCTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabarapl2 (93739)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor-associated protein-like 2
Transcript:
RefSeq NM_026693.4 (NON-CURRENT)
Match location:
Position 481 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCTGCTTGTGTCTCTTGTA
Hairpin Sequence:
5'-CCGG-TTCCTGCTTGTGTCTCTTGTA-CTCGAG-TACAAGAGACACAAGCAGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCTGCTTGTGTCTCTTGTACTCGAGTACAAGAGACACAAGCAGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCTGCTTGTGTCTCTTGTACTCGAGTACAAGAGACACAAGCAGGAA-3'

Other clones with same target sequence:

(none)