Construct: shRNA TRCN0000336523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026693.4-155s21c1
DNA Barcode:
CGCGAAGATCAGAGCGAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabarapl2 (93739)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor-associated protein-like 2
Transcript:
RefSeq NM_026693.4 (NON-CURRENT)
Match location:
Position 155 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGAAGATCAGAGCGAAGTA
Hairpin Sequence:
5'-CCGG-CGCGAAGATCAGAGCGAAGTA-CTCGAG-TACTTCGCTCTGATCTTCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGAAGATCAGAGCGAAGTACTCGAGTACTTCGCTCTGATCTTCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGAAGATCAGAGCGAAGTACTCGAGTACTTCGCTCTGATCTTCGCG-3'

Other clones with same target sequence:

(none)