Construct: shRNA TRCN0000336544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011378.2-1721s21c1
DNA Barcode:
ACCCAAACTGATGAGTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sin3a (20466)
Gene Description:
transcriptional regulator, SIN3A (yeast)
Transcript:
RefSeq NM_011378.2 (CURRENT)
Match location:
Position 1721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCAAACTGATGAGTCTAAA
Hairpin Sequence:
5'-CCGG-ACCCAAACTGATGAGTCTAAA-CTCGAG-TTTAGACTCATCAGTTTGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCAAACTGATGAGTCTAAACTCGAGTTTAGACTCATCAGTTTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCAAACTGATGAGTCTAAACTCGAGTTTAGACTCATCAGTTTGGGT-3'

Other clones with same target sequence:

(none)