Construct: shRNA TRCN0000336605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017552.1-1887s21c1
DNA Barcode:
TCTTATGGATGGATTAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATAD2B (54454)
Gene Description:
ATPase family AAA domain containing 2B
Transcript:
RefSeq NM_017552.1 (NON-CURRENT)
Match location:
Position 1887 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTATGGATGGATTAGATAA
Hairpin Sequence:
5'-CCGG-TCTTATGGATGGATTAGATAA-CTCGAG-TTATCTAATCCATCCATAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTATGGATGGATTAGATAACTCGAGTTATCTAATCCATCCATAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTATGGATGGATTAGATAACTCGAGTTATCTAATCCATCCATAAGA-3'

Other clones with same target sequence:

(none)