Construct: shRNA TRCN0000336667

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001143967.1-1182s21c1
DNA Barcode:
GGTTGTCAGTGCTGCGTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFCAB8 (388795)
Gene Description:
EF-hand calcium binding domain 8
Transcript:
RefSeq NM_001143967.1 (CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336667)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTGTCAGTGCTGCGTTTAA
Hairpin Sequence:
5'-CCGG-GGTTGTCAGTGCTGCGTTTAA-CTCGAG-TTAAACGCAGCACTGACAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTGTCAGTGCTGCGTTTAACTCGAGTTAAACGCAGCACTGACAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTGTCAGTGCTGCGTTTAACTCGAGTTAAACGCAGCACTGACAACC-3'

Other clones with same target sequence:

(none)