Construct: shRNA TRCN0000336757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146339.1-258s21c1
DNA Barcode:
CCGGAGCAAGGTAACAAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VSTM2B (342865)
Gene Description:
V-set and transmembrane domain containing 2B
Transcript:
RefSeq NM_001146339.1 (CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGCAAGGTAACAAATAA
Hairpin Sequence:
5'-CCGG-CCGGAGCAAGGTAACAAATAA-CTCGAG-TTATTTGTTACCTTGCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGCAAGGTAACAAATAACTCGAGTTATTTGTTACCTTGCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGCAAGGTAACAAATAACTCGAGTTATTTGTTACCTTGCTCCGG-3'

Other clones with same target sequence:

(none)