Construct: shRNA TRCN0000336783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077440.1-1736s21c1
DNA Barcode:
CCTGAGCAGGTAAAGTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCLAF1 (9774)
Gene Description:
BCL2 associated transcription factor 1
Transcript:
RefSeq NM_001077440.1 (CURRENT)
Match location:
Position 1736 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGCAGGTAAAGTCTGAA
Hairpin Sequence:
5'-CCGG-CCTGAGCAGGTAAAGTCTGAA-CTCGAG-TTCAGACTTTACCTGCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGCAGGTAAAGTCTGAACTCGAGTTCAGACTTTACCTGCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGCAGGTAAAGTCTGAACTCGAGTTCAGACTTTACCTGCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000220218