Construct: shRNA TRCN0000336792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145117.4-2979s21c1
DNA Barcode:
ACATCCGGACCGATGACATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAV2 (89797)
Gene Description:
neuron navigator 2
Transcript:
RefSeq NM_145117.4 (NON-CURRENT)
Match location:
Position 2979 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATCCGGACCGATGACATTA
Hairpin Sequence:
5'-CCGG-ACATCCGGACCGATGACATTA-CTCGAG-TAATGTCATCGGTCCGGATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATCCGGACCGATGACATTACTCGAGTAATGTCATCGGTCCGGATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATCCGGACCGATGACATTACTCGAGTAATGTCATCGGTCCGGATGT-3'

Other clones with same target sequence:

(none)