Construct: shRNA TRCN0000336898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014828.2-174s21c1
DNA Barcode:
CCGAGACATTCCATACACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOX4 (9878)
Gene Description:
TOX high mobility group box family member 4
Transcript:
RefSeq NM_014828.2 (NON-CURRENT)
Match location:
Position 174 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGACATTCCATACACCAA
Hairpin Sequence:
5'-CCGG-CCGAGACATTCCATACACCAA-CTCGAG-TTGGTGTATGGAATGTCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGACATTCCATACACCAACTCGAGTTGGTGTATGGAATGTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGACATTCCATACACCAACTCGAGTTGGTGTATGGAATGTCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000084519
  2. TRCN0000140848