Construct: shRNA TRCN0000336939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145026.1-6152s21c1
DNA Barcode:
GTGTTCCAGGTTCGGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRQ (374462)
Gene Description:
protein tyrosine phosphatase receptor type Q
Transcript:
RefSeq NM_001145026.1 (NON-CURRENT)
Match location:
Position 6152 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTCCAGGTTCGGATTATA
Hairpin Sequence:
5'-CCGG-GTGTTCCAGGTTCGGATTATA-CTCGAG-TATAATCCGAACCTGGAACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTCCAGGTTCGGATTATACTCGAGTATAATCCGAACCTGGAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTCCAGGTTCGGATTATACTCGAGTATAATCCGAACCTGGAACAC-3'

Other clones with same target sequence:

(none)