Construct: shRNA TRCN0000336959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145715.1-1401s21c1
DNA Barcode:
GCTGCATGAGAACCGTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KPNA7 (402569)
Gene Description:
karyopherin subunit alpha 7
Transcript:
RefSeq NM_001145715.1 (NON-CURRENT)
Match location:
Position 1401 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATGAGAACCGTCAAAT
Hairpin Sequence:
5'-CCGG-GCTGCATGAGAACCGTCAAAT-CTCGAG-ATTTGACGGTTCTCATGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATGAGAACCGTCAAATCTCGAGATTTGACGGTTCTCATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATGAGAACCGTCAAATCTCGAGATTTGACGGTTCTCATGCAGC-3'

Other clones with same target sequence:

(none)