Construct: shRNA TRCN0000337042

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017650.2-1588s21c1
DNA Barcode:
GATATGGAGTACTCGGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R9A (55607)
Gene Description:
protein phosphatase 1 regulatory subunit 9A
Transcript:
RefSeq NM_017650.2 (CURRENT)
Match location:
Position 1588 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337042)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATGGAGTACTCGGAAATT
Hairpin Sequence:
5'-CCGG-GATATGGAGTACTCGGAAATT-CTCGAG-AATTTCCGAGTACTCCATATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATGGAGTACTCGGAAATTCTCGAGAATTTCCGAGTACTCCATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATGGAGTACTCGGAAATTCTCGAGAATTTCCGAGTACTCCATATC-3'

Other clones with same target sequence:

(none)