Construct: shRNA TRCN0000337086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018206.4-924s21c1
DNA Barcode:
CCTCCAGACTTTGAATCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS35 (55737)
Gene Description:
VPS35 retromer complex component
Transcript:
RefSeq NM_018206.4 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAGACTTTGAATCCTTT
Hairpin Sequence:
5'-CCGG-CCTCCAGACTTTGAATCCTTT-CTCGAG-AAAGGATTCAAAGTCTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAGACTTTGAATCCTTTCTCGAGAAAGGATTCAAAGTCTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAGACTTTGAATCCTTTCTCGAGAAAGGATTCAAAGTCTGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000155666