Construct: shRNA TRCN0000337141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001144013.1-2224s21c1
DNA Barcode:
TTAGATGACAGTGATTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD3 (653489)
Gene Description:
RANBP2 like and GRIP domain containing 3
Transcript:
RefSeq NM_001144013.1 (CURRENT)
Match location:
Position 2224 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAGATGACAGTGATTCAAAT
Hairpin Sequence:
5'-CCGG-TTAGATGACAGTGATTCAAAT-CTCGAG-ATTTGAATCACTGTCATCTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAGATGACAGTGATTCAAATCTCGAGATTTGAATCACTGTCATCTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAGATGACAGTGATTCAAATCTCGAGATTTGAATCACTGTCATCTAA-3'

Other clones with same target sequence:

(none)