Construct: shRNA TRCN0000337143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_002346545.1-599s21c1
DNA Barcode:
ATCCTTTCTCAACTTTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GMNC (647309)
Gene Description:
geminin coiled-coil domain containing
Transcript:
RefSeq XM_002346545.1 (NON-CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCTTTCTCAACTTTCAAAT
Hairpin Sequence:
5'-CCGG-ATCCTTTCTCAACTTTCAAAT-CTCGAG-ATTTGAAAGTTGAGAAAGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCTTTCTCAACTTTCAAATCTCGAGATTTGAAAGTTGAGAAAGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCTTTCTCAACTTTCAAATCTCGAGATTTGAAAGTTGAGAAAGGAT-3'

Other clones with same target sequence:

(none)