Construct: shRNA TRCN0000337297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145442.1-992s21c1
DNA Barcode:
TGTTGTGGATCGGCAAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEM (641455)
Gene Description:
POTE ankyrin domain family member M
Transcript:
RefSeq NM_001145442.1 (CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGTGGATCGGCAAGTATA
Hairpin Sequence:
5'-CCGG-TGTTGTGGATCGGCAAGTATA-CTCGAG-TATACTTGCCGATCCACAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGTGGATCGGCAAGTATACTCGAGTATACTTGCCGATCCACAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGTGGATCGGCAAGTATACTCGAGTATACTTGCCGATCCACAACA-3'

Other clones with same target sequence:

(none)