Construct: shRNA TRCN0000337319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146210.1-547s21c1
DNA Barcode:
GGAGGTGGTGGATGATGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPDYE6 (729597)
Gene Description:
speedy/RINGO cell cycle regulator family member E6
Transcript:
RefSeq NM_001146210.1 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGGTGGTGGATGATGAAAT
Hairpin Sequence:
5'-CCGG-GGAGGTGGTGGATGATGAAAT-CTCGAG-ATTTCATCATCCACCACCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGGTGGTGGATGATGAAATCTCGAGATTTCATCATCCACCACCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGGTGGTGGATGATGAAATCTCGAGATTTCATCATCCACCACCTCC-3'

Other clones with same target sequence:

(none)