Construct: shRNA TRCN0000337445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146041.1-847s21c1
DNA Barcode:
TGGCACCAAATGTGAATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRTAP4-9 (100132386)
Gene Description:
keratin associated protein 4-9
Transcript:
RefSeq NM_001146041.1 (CURRENT)
Match location:
Position 847 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCACCAAATGTGAATTAAT
Hairpin Sequence:
5'-CCGG-TGGCACCAAATGTGAATTAAT-CTCGAG-ATTAATTCACATTTGGTGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCACCAAATGTGAATTAATCTCGAGATTAATTCACATTTGGTGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCACCAAATGTGAATTAATCTCGAGATTAATTCACATTTGGTGCCA-3'

Other clones with same target sequence:

(none)