Construct: shRNA TRCN0000337472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010546.2-1725s21c1
DNA Barcode:
GCATCTAGTAGAGCGGATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ikbkb (16150)
Gene Description:
inhibitor of kappaB kinase beta
Transcript:
RefSeq NM_010546.2 (CURRENT)
Match location:
Position 1725 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTAGTAGAGCGGATGAT
Hairpin Sequence:
5'-CCGG-GCATCTAGTAGAGCGGATGAT-CTCGAG-ATCATCCGCTCTACTAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTAGTAGAGCGGATGATCTCGAGATCATCCGCTCTACTAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTAGTAGAGCGGATGATCTCGAGATCATCCGCTCTACTAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000026913