Construct: shRNA TRCN0000337535
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_138314.3-997s21c1
- DNA Barcode:
- GCCAGAGAAATGGAATTGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nme7 (171567)
- Gene Description:
- NME/NM23 family member 7
- Transcript:
- RefSeq NM_138314.3 (NON-CURRENT)
- Match location:
- Position 997 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000337535)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGAGAAATGGAATTGTTT
- Hairpin Sequence:
-
5'-CCGG-GCCAGAGAAATGGAATTGTTT-CTCGAG-AAACAATTCCATTTCTCTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGAGAAATGGAATTGTTTCTCGAGAAACAATTCCATTTCTCTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGAGAAATGGAATTGTTTCTCGAGAAACAATTCCATTTCTCTGGC-3'
Other clones with same target sequence:
-
TRCN0000024705
