Construct: shRNA TRCN0000337575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019400.2-2748s21c1
DNA Barcode:
GCTATTCTGAATGATACCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rabep1 (54189)
Gene Description:
rabaptin, RAB GTPase binding effector protein 1
Transcript:
RefSeq NM_019400.2 (NON-CURRENT)
Match location:
Position 2748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTCTGAATGATACCAAA
Hairpin Sequence:
5'-CCGG-GCTATTCTGAATGATACCAAA-CTCGAG-TTTGGTATCATTCAGAATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTCTGAATGATACCAAACTCGAGTTTGGTATCATTCAGAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTCTGAATGATACCAAACTCGAGTTTGGTATCATTCAGAATAGC-3'

Other clones with same target sequence:

  1. TRCN0000191959