Construct: shRNA TRCN0000337642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007771.3-2009s21c1
DNA Barcode:
GCAAGCAGACTGAATATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cry1 (12952)
Gene Description:
cryptochrome 1 (photolyase-like)
Transcript:
RefSeq NM_007771.3 (CURRENT)
Match location:
Position 2009 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCAGACTGAATATTGAA
Hairpin Sequence:
5'-CCGG-GCAAGCAGACTGAATATTGAA-CTCGAG-TTCAATATTCAGTCTGCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCAGACTGAATATTGAACTCGAGTTCAATATTCAGTCTGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCAGACTGAATATTGAACTCGAGTTCAATATTCAGTCTGCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000175971