Construct: shRNA TRCN0000337750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021328.3-1353s21c1
DNA Barcode:
GCTGTCCACTAGGAAATGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bin3 (57784)
Gene Description:
bridging integrator 3
Transcript:
RefSeq NM_021328.3 (CURRENT)
Match location:
Position 1353 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCCACTAGGAAATGTTA
Hairpin Sequence:
5'-CCGG-GCTGTCCACTAGGAAATGTTA-CTCGAG-TAACATTTCCTAGTGGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCCACTAGGAAATGTTACTCGAGTAACATTTCCTAGTGGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCCACTAGGAAATGTTACTCGAGTAACATTTCCTAGTGGACAGC-3'

Other clones with same target sequence:

  1. TRCN0000180820