Construct: shRNA TRCN0000337775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024475.5-983s21c1
DNA Barcode:
AGCTCATCTAAACCGTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ublcp1 (79560)
Gene Description:
ubiquitin-like domain containing CTD phosphatase 1
Transcript:
RefSeq NM_024475.5 (CURRENT)
Match location:
Position 983 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTCATCTAAACCGTGATAA
Hairpin Sequence:
5'-CCGG-AGCTCATCTAAACCGTGATAA-CTCGAG-TTATCACGGTTTAGATGAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTCATCTAAACCGTGATAACTCGAGTTATCACGGTTTAGATGAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTCATCTAAACCGTGATAACTCGAGTTATCACGGTTTAGATGAGCT-3'

Other clones with same target sequence:

(none)