Construct: shRNA TRCN0000337782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022995.3-1462s21c1
DNA Barcode:
GTTGAGCTCTGTGCGTGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pmepa1 (65112)
Gene Description:
prostate transmembrane protein, androgen induced 1
Transcript:
RefSeq NM_022995.3 (CURRENT)
Match location:
Position 1462 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGAGCTCTGTGCGTGAATG
Hairpin Sequence:
5'-CCGG-GTTGAGCTCTGTGCGTGAATG-CTCGAG-CATTCACGCACAGAGCTCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGAGCTCTGTGCGTGAATGCTCGAGCATTCACGCACAGAGCTCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGAGCTCTGTGCGTGAATGCTCGAGCATTCACGCACAGAGCTCAAC-3'

Other clones with same target sequence:

(none)