Construct: shRNA TRCN0000337854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025656.4-578s21c1
DNA Barcode:
TTCCTCCTTTGCTTAGTATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gemin2 (66603)
Gene Description:
gem nuclear organelle associated protein 2
Transcript:
RefSeq NM_025656.4 (NON-CURRENT)
Match location:
Position 578 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCTCCTTTGCTTAGTATTG
Hairpin Sequence:
5'-CCGG-TTCCTCCTTTGCTTAGTATTG-CTCGAG-CAATACTAAGCAAAGGAGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCTCCTTTGCTTAGTATTGCTCGAGCAATACTAAGCAAAGGAGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCTCCTTTGCTTAGTATTGCTCGAGCAATACTAAGCAAAGGAGGAA-3'

Other clones with same target sequence:

(none)