Construct: shRNA TRCN0000337873
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_026443.4-948s21c1
- DNA Barcode:
- GCAGAACCCTTAGAGGTTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mtfp1 (67900)
- Gene Description:
- mitochondrial fission process 1
- Transcript:
- RefSeq NM_026443.4 (CURRENT)
- Match location:
- Position 948 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000337873)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGAACCCTTAGAGGTTAAA
- Hairpin Sequence:
-
5'-CCGG-GCAGAACCCTTAGAGGTTAAA-CTCGAG-TTTAACCTCTAAGGGTTCTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGAACCCTTAGAGGTTAAACTCGAGTTTAACCTCTAAGGGTTCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGAACCCTTAGAGGTTAAACTCGAGTTTAACCTCTAAGGGTTCTGC-3'
Other clones with same target sequence:
(none)
