Construct: shRNA TRCN0000338051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010785.2-863s21c1
DNA Barcode:
GACTGAATACAAGCGAAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mdm1 (17245)
Gene Description:
transformed mouse 3T3 cell double minute 1
Transcript:
RefSeq NM_010785.2 (CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGAATACAAGCGAAATTT
Hairpin Sequence:
5'-CCGG-GACTGAATACAAGCGAAATTT-CTCGAG-AAATTTCGCTTGTATTCAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGAATACAAGCGAAATTTCTCGAGAAATTTCGCTTGTATTCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGAATACAAGCGAAATTTCTCGAGAAATTTCGCTTGTATTCAGTC-3'

Other clones with same target sequence:

(none)