Construct: shRNA TRCN0000338118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026312.4-304s21c1
DNA Barcode:
CCAGAAGAACTCAAATCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbdc1 (67683)
Gene Description:
polysaccharide biosynthesis domain containing 1
Transcript:
RefSeq NM_026312.4 (NON-CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGAACTCAAATCAGAA
Hairpin Sequence:
5'-CCGG-CCAGAAGAACTCAAATCAGAA-CTCGAG-TTCTGATTTGAGTTCTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGAACTCAAATCAGAACTCGAGTTCTGATTTGAGTTCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGAACTCAAATCAGAACTCGAGTTCTGATTTGAGTTCTTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000177650