Construct: shRNA TRCN0000338128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019869.2-441s21c1
DNA Barcode:
CATCGAGTGTGACGTGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm14 (56275)
Gene Description:
RNA binding motif protein 14
Transcript:
RefSeq NM_019869.2 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGTGTGACGTGGTAAA
Hairpin Sequence:
5'-CCGG-CATCGAGTGTGACGTGGTAAA-CTCGAG-TTTACCACGTCACACTCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGTGTGACGTGGTAAACTCGAGTTTACCACGTCACACTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGTGTGACGTGGTAAACTCGAGTTTACCACGTCACACTCGATG-3'

Other clones with same target sequence:

(none)