Construct: shRNA TRCN0000338164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002228.3-1950s21c1
DNA Barcode:
GAACAGGTGGCACAGCTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JUN (3725)
Gene Description:
Jun proto-oncogene, AP-1 transcription factor subunit
Transcript:
RefSeq NM_002228.3 (NON-CURRENT)
Match location:
Position 1950 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGGTGGCACAGCTTAAA
Hairpin Sequence:
5'-CCGG-GAACAGGTGGCACAGCTTAAA-CTCGAG-TTTAAGCTGTGCCACCTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGGTGGCACAGCTTAAACTCGAGTTTAAGCTGTGCCACCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGGTGGCACAGCTTAAACTCGAGTTTAAGCTGTGCCACCTGTTC-3'

Other clones with same target sequence:

(none)