Construct: shRNA TRCN0000338222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003948.3-564s21c1
DNA Barcode:
GAGTTATGGAATGGTGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL2 (8999)
Gene Description:
cyclin dependent kinase like 2
Transcript:
RefSeq NM_003948.3 (NON-CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTATGGAATGGTGATGAA
Hairpin Sequence:
5'-CCGG-GAGTTATGGAATGGTGATGAA-CTCGAG-TTCATCACCATTCCATAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTATGGAATGGTGATGAACTCGAGTTCATCACCATTCCATAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTATGGAATGGTGATGAACTCGAGTTCATCACCATTCCATAACTC-3'

Other clones with same target sequence:

  1. TRCN0000000726