Construct: shRNA TRCN0000338253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004723.3-365s21c1
DNA Barcode:
TCCGTTTCTCTTCGAAGTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF2 (9181)
Gene Description:
Rho/Rac guanine nucleotide exchange factor 2
Transcript:
RefSeq NM_004723.3 (CURRENT)
Match location:
Position 365 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGTTTCTCTTCGAAGTAAG
Hairpin Sequence:
5'-CCGG-TCCGTTTCTCTTCGAAGTAAG-CTCGAG-CTTACTTCGAAGAGAAACGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGTTTCTCTTCGAAGTAAGCTCGAGCTTACTTCGAAGAGAAACGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGTTTCTCTTCGAAGTAAGCTCGAGCTTACTTCGAAGAGAAACGGA-3'

Other clones with same target sequence:

(none)